Sentence examples for protected footprint from inspiring English sources

Therefore, the length of the DNA fragments provides no information as to the location of the minimally protected footprint and as such, single end sequencing is appropriate for conventional ChIP-seq.

Overall, this shows that using a high-resolution ChIP technique to map the protected footprint of PolII achieves comparable resolution to the single base-pair resolution achieved by mapping the position of the active site of PolII via nascent chain mapping.

Ribosome protected footprints were generated through nuclease treatment of cell extract in the presence of cycloheximide.

Ribosome profiling experiments include the following major stages: cells are treated with cycloheximide (for example) to arrest translating ribosomes; then, RNA fragments protected by ribosomes from RNases are isolated and processed for high-throughput sequencing, resulting in reads of ribosomes protected footprints.

Due to the sonication bias, it is clear that the sonicated fragments do not represent the minimally protected DNA footprint, and as such the length of the recovered DNA fragments provides no information.

Until this work, only the antisense part of this potential E-box was reported to be protected in footprinting experiments in vivo [ 18, 22], because the sense strand cannot be analyzed due to sequence extension in the linker-mediated PCR is fully determined at the TATA-box (unpublished data, Würleitner E. and Mach R.L).

But it is unclear whether protecting the footprint is politically or morally necessary.

"In that case, it would protect every footprint from Neil and Buzz and all the flight hardware," Mr. Kelso said.

This approach relies on the ability of the translating ribosome to protect a footprint of ~28 nt from RNase digestion (see Additional file 2: Figure S1).

The authors of the method used a nuclease protection assay to establish that, in yeast treated with the elongation inhibitor cycloheximide, each ribosome protects a footprint of 28 nucleotides (nt), confirming earlier reports (Steitz, 1969; Wolin and Walter, 1988).

Protecting those footprints was a remarkable achievement, the result of true aesthetic vision and popular will.