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It is involved in the degradation of proteins, which is an essential process within the cell [ 7]; the nuclear pore complex is responsible for the protected exchange of components between the nucleus and cytoplasm and for preventing the transport of material not destined to cross the nuclear membrane [ 8].
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The banks that run the dark pools have pressed for an end to the rules that protect exchanges.
Moreover, helices in close contact with the hydrophobic core of the T domain (e.g., TH3 (residues 241 249, 250 255) and TH5 (residues 281−284)) are also highly protected from exchange, and the maximal exchange is less than 5% after 1 h of incubation.
First, we used hydrogen deuterium exchange mass spectrometry (HDX MS) to identify regions on SecA that are protected from exchange upon dimerization and to probe conformational changes associated with dimerization.
Most of the archaic rules that protected the exchange from competition have gone.
He protected the exchange's open-cry auction system, which many deemed outdated and rife with human error, against the near universal move to all-electronic trading.
After 45 h, 8 amide protons were still protected from exchange (Supplementary Table S2).
As expected, some regions in the dimer became more protected against exchange than in the monomer.
Water accessibility studies show that regions in both N- and C-termini are protected from exchange and that the C-terminus has less flexibility.
Additional residues lying within the preprotein binding domain and the C-terminus are also protected from exchange upon dimerization, indicating linkage to a conformational transition of the preprotein binding domain from an open to a closed state.
The NLS was not resolved in a crystal of the protein, but HDX MS showed that the NLS was protected from exchange in the F383 V mutant versus the wild-type.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com