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Perhaps, this was because the CSC were protected (as described) from the soluble TME in the tumor conglomerates that are evidenced in MPE.
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The proline derivatives 3– 7 were Fmoc-protected as described in literature (Ruan et al. 1991; Albericio 2002) and incorporated in the respective peptide sequences.
Now, tongue as described.
After 1 day of incubation, the crystals were cryo-protected and frozen as described (Bae et al., 2015).
Hybridisations were carried out at 45°C for 14 h after denaturation at 65°C for 15 min. S1 nuclease digestions and analyses of RNA-protected fragments were performed as described previously [ 54].
Following washing as described below, Fmoc-protected aminocaproic acid (3 equiv) was then preactivated with PyBop (3 equiv) and TEA (6 equiv) in DMF (≥0.1 M) for 1 h with Ar bubbling.
Adult normal human skin samples were obtained by punch biopsy (4 mm) from sun-protected (hip/buttocks) areas of healthy adults (30 40 years), as described previously (Quan et al., 2010).
Once isolated, they were resuspended in THF and treated with n-BuLi at −78 °C to generate the Wittig ylids, as described by Plettenburg et al. Subsequently, the protected lyxose derivative 6, which was synthesised as previously described, was condensed with the ylids to afford the desired olefins 7, 8 and 9 as a mixture of cis and trans isomers (Scheme 1).
This compound was straightforwardly synthesized by coupling silyl-protected 11-bromoundecanol to 2- 2-ethoxyethoxy)ethanol followed by deprotection and oxidation of the alcohol to the aldehyde, as described in the Supporting Information.
as described previously [27].
Optionally, subsequent uncaging of disulfide-protected Cys residues is achieved by treatment with DTT or TCEP (500 μ m) for 15 min. Exchanged monomers were used for tetramerisation as previously described 20 or for cytokine release assays as described below.
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