Herein we describe how we solved these technical challenges and used a functional approach to identify hit sequences that protect an IL3-dependent cell line from IL3 withdrawal, and how we hit-optimized by random mutagenesis and re-screening.
However, essential genes need to be stable and to be protected in the sequence.
Therefore, laboratories can be protected from sequencing amplicons that may interfere with real-time PCR diagnostics.
The CENH3 nucleosome specific sequence was not revealed until the 147 bp micrococcal nuclease protected DNA sequences were precisely aligned.
The data processing system is protected through parity sequences specified by a high rate real convolution code.
In the case of α-aminododecanoic acid (Adod), however, two preparations of the enantiopure α-amino acid donor have been described and are outlined in Fig. 2. Beginning from protected α-amino malonate 39, a sequence of alkylation hydrolysis decarboxylation leads to N-Boc-Adod in racemic form.
77 78 In contrast, blinding seeks to prevent performance and detection bias by protecting the sequence after assignment.
2 In contrast, blinding seeks to prevent performance and ascertainment bias, protects the sequence after allocation, and cannot always be implemented.
Firstly, TFs bound with hairpin probes and protected signal complementary sequences against cleavage by Nt.BbvCI due to space hindrance effect, while the excess hairpin probes were effectively digested to avoid false positive signal.
We asked the question whether these barrier proteins protected the (ChβGI 2 sequences from methylation in the male germ line.
The position of the two confirmed xrRNAs is consistent with a role in protecting downstream sequences and structures, including the DBs and 3′SL from degradation.
