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The bins were covered with jute cloth to protect from light and predators.
All solutions were wrapped in aluminum foil to protect from light.
In protoplast transient assay, isolated protoplasts were kept in the dark condition to protect from light damage, thus the GFP fluorescence can be observed in the vacuoles..Consequently, this result suggested that OsVPE3 is a vacuole-targeting protein similar to OsVPE1.
The oil was stored in a refrigerator at 4°C to protect from light and degeneration.
The stock was aliquoted in dark-coloured glass vials with screw tops (Agilent Technologies, Wokingham, UK) to protect from light and oxidation and stored at −20°C for up to 6 months.
Briefly, cells were washed twice with PBS and incubated to protect from light for 30 min at room temperature in the presence of 2 μM calcein-AM solution prepared in PBS.
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The solution was protected from light.
All experimental steps involving RES were protected from light.
Samples were protected from light at all times.
The stock solutions were freshly prepared and protected from light.
The stock solutions were stored at 4 °C, protected from light.
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