Several recombinant, viral vectors have been used in human trials (Table 3) – of which adenovirus, herpes simplex virus, vaccinia and poxviruses give only transient transgene expression, so attention has focused on retroviruses and adeno-associated virus (AAV), which offer the prospect of long-term expression.
This opens the prospect of manipulating gene expression and/or signalling activities in vivo, simultaneously in HSCs and niche cells, and decrypting their reciprocal communication under physiological and pathological conditions.
Moreover, these data provided our group with the prospect of exploiting TSP-1 expression or plasma levels as pharmacodynamic markers for CPT-11 metronomic chemotherapy clinical regimens, as previously suggested by Kerbel and Kamen (2004).
The putative connection with chromatin raises the fascinating prospect of a functional link between expression noise and epigenetic gene silencing.
Moreover, given that sCD59 is already found naturally [39] in ocular tissues (albeit not likely at the levels following recombinant transgene expression) the prospect of an immune response against sCD59 is further diminished.
Recent findings from microarray studies have raised the prospect of a standardized diagnostic gene expression platform to enhance accurate diagnosis and risk stratification in paediatric acute lymphoblastic leukaemia (ALL).
Having isolated metagenomic DNA, two complementary approaches can be used for prospecting novel enzymes from it; function-based screening of expression libraries and sequence-based gene searches.
The possible effects of SNPs at HLA-C and HLA-G 3′ UTRs on miRNA binding were discussed as well as the prospects of using miRNA expression signatures as a biomarker of disease progression.
The findings from this and other studies raised the prospect of developing a standardized diagnostic gene expression platform to enhance accurate diagnosis and risk stratification.
The prospect of repowering the two plants brought expressions of delight from environmentalists and public officials, including Senator Charles E. Schumer, Democrat of New York.
We conclude that the appropriate design of expression vectors and MGMT protein features will be crucial for the long-term prospects of this promising selection principle.
