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ZONING: Two-acre residential PROS: Complex of 14 industrial buildings, including historic Tesla laboratory.
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Internalization of the FE-Pro complex was visualized with electron microscopy using standard techniques.
No residual extracellular FE-Pro complex was observed in the labeled samples following the final PBS/heparin wash.
Prussian blue staining of Fe-Pro labeled mouse or human BMSCs revealed abundant uptake of the Fe-Pro complex into the cytoplasm in approximately 100% of cells.
The solution was agitated for 1 min to allow the FE-Pro complex to form and was immediately added to adherent NSCs (80% confluence).
Transmission EM micrographs showed the FE-Pro complex as an electron-dense structure in the cytoplasm of labeled NSCs (Fig. 2B).
Adherent NSCs in a 96-well culture plate were labeled at various concentrations of FE-Pro complex (FE at 50, 100, 200 and 250 µg/ml, each of which was complexed with increasing Pro at 3, 10, and 25 µg/ml).
The dengue NS3 was detected at lower levels in Phyllanthus treated infected cells, and this directly reduced the amount of NS2B/NS3 pro complex formed as was observed in this study.
The iron-cores of SPIOs were localized to membrane-bound structures (red arrows; Fig. 2C), indicating that FE-Pro complexes were encapsulated rather than dispersed throughout the cytoplasm.
To determine if the FE-Pro complexes were well-retained and encapsulated within NSCs after implantation into the mouse brain and subsequent tumor-tropic migration, the brain samples were analyzed with TEM and immunohistochemistry.
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