Sentence examples for proprietary sequencing from inspiring English sources
Sanger sequencing of the 3' ends of clones was performed by Agencourt Biosciences (MA, USA), using a proprietary sequencing primer.
Following PCR amplification, these mate-pair libraries are then used as templates in emulsion PCR reactions using SOLiD™ proprietary sequencing beads to generate clonal single molecule templated beads.
The ligation product was reverse-transcribed into cDNA, which was then amplified by 15 PCR cycles and subjected to Illumina's Solexa proprietary sequencing.
Although the presence of these side products can often be discerned by analysis of the product melting curve, opportunities to optimize the primers are limited due to the short length of the adaptor sequences and the specific nucleotide sequences required for compatibility with proprietary sequencing reagents.
The promise for bioinformatics is that public genome data, mixed with proprietary sequence data, clinical data from previous drug efforts and other stores of information, could unearth clues about possible candidates for future drugs.Unlike banking, bioinformatics offers big challenges for data mining because of the greater complexity of the information and processes.
The cDNA was amplified with 18 PCR cycles to produce libraries that were sequenced using Solexa's proprietary sequencing-by-synthesis method.
The cDNA was amplified using the appropriate PCR cycles to produce sequencing libraries that were subjected to Illumina Genome Analyzer's proprietary sequencing-by-synthesis method.
Whole-genome sequencing was performed (CG) using their proprietary sequencing-by-ligation technology [ 10].
The purified PCR products were subjected to Solexa's proprietary sequencing-by-synthesis method on the Illumina 1G Genome Analyzer.
The libraries were quantified by ECO (Illumina, San Diego, USA) and sequenced using the Solexa's proprietary sequencing-by-synthesis method.
The PCR products resolved in a 12% TBE urea polyacrylamide gel were eluted with elution buffer, quantified and subjected to Solexa's proprietary sequencing-by-synthesis method.
