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A proprietary medium access control is developed based on the low-power SimpliciTI radio stack (Texas Instruments Incorporated, San Diego, CA, USA).
Cells are cultured in a proprietary medium by apceth and passaged using Trypsin.
Primary human hepatocytes were obtained from Celsis (Brussels, Belgium), and mRNA was extracted after 24-h culture in proprietary medium (InVitroGRO; Celsis).
Other reports of Vero cultivation in different SFM typically achieved less than 2 × 106 cells/ml [ 35, 43, 44, 46], except one using a proprietary medium [ 45].
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The human acini were shipped in a bottle of proprietary transport medium, which helps preserve functionality/viability.
Briefly, preadipocytes were cultured to confluence in DMEM supplemented with 10% FCS and differentiation induced by incubation with proprietary differentiation medium (Zen-Bio) for 7 days.
These considerations eventually lead to the design of an on-site whole-blood culture, performed in a peculiar type of syringe, as well as a proprietary culture medium, allowing the culture of leukoyctes without needing a CO2 incubator.
Fresh hepatocytes were seeded by the manufacturer (Celsis Inc., Baltimore, MD, USA) at a seeding density of 160,000 cells/cm and were shipped overnight at 4°C, pre-plated on a 12-well plate with the cell cultures immersed in a proprietary "shipping medium".
Plastic adherent MSC are expanded in a proprietary, xenofree medium while non-adherent hematopoietic cells are lost over time due to media exchanges (This described cultivation process is currently also used to generate native, unmodified form MSC ("MSC-Apceth") for another clinical trial (EudraCT-No.: 2010- 021821–10)).
Cells were then cultured with proprietary adipose maintenance medium (Zen-Bio) for an additional 7 days.
These cultures were maintained in 250 mL Erlenmeyer flasks containing 100 ml of RAC-1, a proprietary fresh water medium.
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