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Samples were prepared for sequencing using proprietary kits at Illumina, Inc (CA, USA).
DNA recovery from agarose was done using proprietary kits from Qiagen (Qiagen, CA, USA).
Total RNA samples from leaf, root, stem and callus were processed using proprietary kits at Illumina, Inc. in Hayward (CA, USA).
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Immunohistochemistry was carried out by standard methods on a DAKO Autostainer (Dako, Ely, UK), using its proprietary kit, the DAKO Envision FLEX/HRP Detection System.
The extent of cell death was established by assessing LDH activity in the medium by using a proprietary kit.
For each gene, a number of colonies were selected and up to 20 μg of plasmid DNA was isolated from each culture using a proprietary kit (Qiagen).
BAC clones were grown in LB broth containing chloramphenicol (12.5 μg/ml) and DNA was purified using a proprietary kit (NucleoBond® BAC 100, Macherey-Nagel).
RNA isolates with an RNA Integrity Number (RIN) above 7.0 were sent for polyadenylation selected RNA library preparation with a proprietary kit and high throughput sequencing at the New York Genome Center.
Muscles were sectioned (10 μm) using a cryostat and post-fixed in 4% (w/v) paraformaldehyde prior to staining with hematoxylin and eosin (H & E), or Picrosirius Red using a proprietary kit (Polyscience, Warrington, PA) following the manufacturer's instructions.
10 Findings from a large clinical trial of compression treatment, however, indicated that few patients laundered and reused their short stretch bandages.w1 The four layer bandage, commonly available as a proprietary kit, is designed to be discarded after a single use.
The other three methods, F, D and N, were based on proprietary extraction kits.
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CEO of Professional Science Editing for Scientists @ prosciediting.com