We followed the instructions the proprietary lease required.
McGonigal goes further, though, suggesting that her more purely gameful instructions — the proprietary parts of her method — are just as demonstrably beneficial.
Plasma levels of the following hormones were measured according to the manufacturers' instructions using proprietary ELISA kits from the specified companies: leptin (ELISA-Linco), insulin (ELISA-Linco).
Neurodegeneration in the TH+ cells was visualized by silver staining (FD Neurotechnologies, Ellicott City, MD) according to the manufacturer's instructions using proprietary compounds after immunostaining the sections with antibody against tyrosine hydroxylase (TH, Chemicon, 1∶500) visualized with Vector Blue alkaline phosphatase (Vector labs).
The protein concentration of the supernatant was measured by BCA assay (Fisher Scientific, Loughborough, UK) and reactions were performed according to manufacturer's instructions using proprietary supercoiled DNA and 1 μl of test extract.
The alternative If you choose not to garden organically, buy a proprietary weedkiller and follow the instructions to the letter.
Preparation of protein samples, labeling, application to the Panorama® Cell Signaling Array chip and data analysis was performed according to manufacturers' instructions using the proprietary solutions and equipment provided (Panorama® Cell Signaling Antibody Microarray, Sigma, St . Louis MO.
Cluster formation, primer hybridization, and singled-end 76 cycle sequencing were performed on an Illumina Genome Analyzer IIx using proprietary reagents according to the manufacturer's instructions.
pTP-mi5 levels were determined with a custom-designed TaqMan small RNA assay (proprietary to Life Technologies Austria, Vienna, Austria) according to the instructions of the manufacturer.
RNAs ranging from 18 to 30 nt were then purified from a 15% denaturing polyacrylamide gel and sequentially ligated to proprietary adapters according to the manufacturer's instructions (Illumina Inc) before sequencing.
