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Tissues were subjected to Heat Induced Epitope Retrieval (HIER) using an automated antigen-retrieval system (Retriever™) and a proprietary buffer solution (DakoCytomation Target Retrieval Solution, DAKO, Hamburg, Germany).
To enhance detection, tissues were subjected to Heat Induced Epitope Retrieval (HIER) using an automated antigen-retrieval system (Retriever™) and a proprietary buffer solution (DakoCytomation Target Retrieval Solution, DAKO, Hamburg, Germany).
The observed differences in amplification profiles are likely due to variation in the proprietary buffer conditions and structural differences between the two DNA polymerase families.
The resulting concentrated cellular material was suspended in proprietary buffer, sedimented onto slides, washed, and stained using the anti-cytokeratin antibody anti-CD45 and DAPI.
Liquid Tissue lysates were directly injected into the LC system with no processing because the proprietary buffer contains no components that may interfere with chromatography or suppress ionization.
We minimized non-specific binding by using fragments (Fab, Fab', or Fab'2) as capture antibodies and by using proprietary buffer reagents (in addition to the standard casein, BSA, and detergents) to minimize non-specific binding to the solid phase.
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We acknowledge that proprietary buffer-assisted rate adaptation for HSPA could exist and be in operation in commercial devices.
Hybridized DNA sample was removed by washing using proprietary buffers.
Slides were washed with Agilent proprietary buffers, dried under nitrogen flow, and scanned using 100% PMT power for both wavelengths.
The slides were washed with Agilent proprietary buffers, dried under a nitrogen flow, and scanned (Agilent, Palo Alto, CA, USA) using 100% PMT (photomultiplier tube) power for both wavelengths.
The flow-through, containing the low-abundance proteins, was collected, and the high-abundance proteins that remained bound to the column were eluted with buffer B at a flow rate of 1 ml/min (buffers A and B are proprietary buffers included with the MARS depletion kit, and their composition is not disclosed by the manufacturer).
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