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The fluorescence experiments suggested that the proposed binding of L1 and Al3+ ion is in a 1 1 stoichiometry.
Furthermore, the previously proposed binding of a third zinc ion close to the active site of IMP-6 mutant S121G was corroborated by our simulations.
Early models of nucleation proposed binding of a single VCA to a single site on Arp2/3 complex.
This may reflect proposed binding of both UL89 and UL56 (Table 1) by this class of molecules.
Figure 13 suggests a possible arrangement illustrating this point that also agrees with the proposed binding of AEBP2 to nucleosomal DNA (Kim et al., 2009).
The soluble gp130 may regulate maspin expression also in the absence of the IL-6 (data not shown) confirming the already proposed binding of sgp130 to other ligands/soluble receptors (Montero-Julian et al. 1997, Jones et al. 2001).
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Although a simplistic model for substrate inhibition proposes binding of two substrate molecules to an enzyme to form an ES2 complex, in many instances, however, substrate inhibition involves a more complex mechanism that includes slow-transition between multiple enzyme species in solution.
X-ray crystallographic studies that confirm the proposed binding mode of this class of inhibitors in p38α and provide evidence for the proposed intramolecular nitrogen sulfur interaction are discussed.
The proposed binding mode of this new class of p38α inhibitors was confirmed by X-ray crystallographic studies of a representative inhibitor (6a) bound to the p38α enzyme.
Rectal biopsy specimens from patients with IBD were shown to have increased methylation of the CpG island closest to the transcriptional start site of IL8RA: the proposed binding site of transcription factor PU.1 (SPI-1).
In POX and CBDH, carbon atoms C2 and C1 of the ligands (site of oxidative attack in the proposed binding mode of the natural substrates) are located at this position (26, 27).
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