The expected relationship representing proportionate increases in expression (slope of 1.0, intercept of 0) is shown in each panel.
An arabinose gradient liquid β-Galactosidase assay exhibited a concomitant decrease in β-Galactosidase activity with a proportionate HCL2 expression, observed by Western blotting with Anti-His6X antibodies, confirming a robust expression of HCL2-His6X peptine in three-hybrid experiments.
Genes COX17, SPA2, and SDH2 showed an increase in expression in the mini_4n strain, however expression remained significantly lower than the expected two-fold difference proportionate to the gene amplification.
Genes in Class 1 show proportionate increases in mRNA abundance as gene copy increases across the strain panel, with a slope of 1.0 and a log2 intercept of 0 that indicates comparable expression in the two euploid strains.
Secondly, there is an expectation of a linear relationship in which an increase in copy number results in a proportionate increase in protein expression; however, this is not always the case [ 12].
This is consistent with occasional cells having sufficient methylation to cause partial loss of allelic expression, and perhaps a proportionate change in risk of neoplastic transformation.
The lack of α-secretase stimulation was expected to be reflected in a lack of C83 production, while significant increases in BACE1 expression and activity were expected to be associated with proportionate changes in C99 production.
In contrast, amplified genes with expression proportionate to gene copy showed distinct enrichment for genes encoding proteins localized to the cytoplasm or to membranes, stress defense proteins, and kinases and transferases (p < 1e-5, Supplementary file 2).
It was not proportionate or in moderation.
"We have a proportionate response in place," he said.
