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In addition, a small proportion of sequencing tags could be mapped to exon sequences, which are likely to be RNA degradation products.
However, a significant proportion of sequencing reads were unannotated in all test samples.
These areas of great coverage "waste" a considerable proportion of sequencing power, making this approach less productive than expected and requiring the collection of many more reads than the minimum necessary to exhaustively cover the entire region under scrutiny.
We evaluated the 'false-discovery' rate of the method for the identification of TSSs (that is, the proportion of sequencing reads not actually mapping to the 5′-end of capped RNAs) by comparing libraries made using samples treated or not with the tobacco acid pyrophosphatase (TAP), which is required for efficient and specific ligation of the biotinylated primer to the 5′-end of capped-RNAs.
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The proportion of sequence PCC voltages to the grid voltage can be illustrated in Fig. 7 under different capacity ratios of the SCBs to the RECS.
Open image in new window Fig. 7 Proportion of sequence PCC voltages to the grid voltage under different capacity ratios of SCBs to the total RECS.
The red solid and dashed lines show the proportion of sequences that have foreshocks for all families and clusters, respectively (right axis).
In Zodletone spring, a significant proportion of sequences belonged to novel and unique lineages (3804 sequences).
Imputation of genetic variation based on a proportion of sequenced samples is feasible.
Thus, coding regions with a greater proportion of sequence overlapping coding fRNAs evolve more slowly.
In all libraries, we noted a high proportion of sequence tags that showed variation from their mature reference sequence.
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