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The proportion of sequence identity to human NAT1 varied from 51.2% (Danio rerio NAT4) to 98.9% (Gorilla gorilla NAT1) at the nucleotide level.
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Because rt here is computed based on the equation 1 − P = e rt and 1 − p is the sequence identity, we plotted the distribution of sequence identity proportions in Figure 1B for both WGD pairs and other paralogous pairs (pairs of paralogs that are the result of a small-scale duplication event).
Two query parameters are adjustable: the proportion of overlapped regions (≥80 100%) and the level of sequence identity (≥60%∼≥90%) between the query sequence(s) in the target species and the matched sequence(s) in the subject species.
Percentage of sequence identity was calculated by counting the proportion of nucleotides in the p73-bound sequences with exact matches in the overlaid genome.
We removed the redundancy of the alignment discarding sequences with more than 63% of sequence identity.
What level of sequence identity is present?
The degree of sequence identity varied substantially.
d This transcript has 97% of sequence identity with gene LmjF31.0900.
These different subunits share from 44%to81%1% of sequence identity.
The degree of sequence identity threshold is a priori selected.
The distribution of sequence identities was similar for both external datasets, although there was a higher proportion of 100% identical matches between our data and the previously reported 454 shotgun sequences, whereas more hits with the IWGSC data were found in the 99.00-99.99% identity range.
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