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Whereas the dose effect curve for the proportion of responsive cells was roughly sigmoidal, the curve for [Ca2+]i response amplitudes appeared biphasic, with markedly greater responses at 25 and 50 µM p, p′-DDE (Fig. 2B and C).
4OHT-responsive clones were identified for each construct; however, I-SceI-ERT generated a higher proportion of responsive clones than I-SceI-ERT2.
The direct inhibition of EGFR by Tyrphostin AG537, and its downstream signal transducer, MKK1 kinase, by UO126 or PD98589 reduces EGF activation of ER, as determined by the proportion of responsive cells in our assays.
Interestingly, the proportion of responsive sites of the MT birds was similar to that of the SD females (fig. 1A; <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0002194.e004.PNG" class= inline-graphic"/> ).
Several features were affected: they had 1) a larger auditory area (larger proportion of responsive sites), 2) a lower proportion of specialized neurons (sites responding to only one stimulus) and 3) a larger proportion of generalist sites (sites responding to all stimuli), associated with tonic, non-selective responses.
Furthermore, recent work by other investigators has also found a dissociation in the effects of imprinting on proportion of responsive neurons to the audiovisual imprinting stimulus and its visual component (Nicol & Horn, Proceedings of the Physiological Society 2009 Cardiff, UK. Available at www.physoc.org/Proceedings: Last Accessed Jan 2011).
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Different measures of responses were made: Proportions of responsive sites that differ according to early experience: these proportions are larger in inexperienced animals [19], [21].
As no differences were evidenced between these two groups (e.g. mean proportions of responsive sites: raised in isolation = 92.10±1.32 and pair raised = 93.37), data from these three birds were pooled (socially deprived birds: SD).
6) Do the authors find a correlation between the magnitude of neural responsivity and/or proportion of CS-responsive neurons with the CS-responses of the mice?
This data is now included in Figure 4 figure supplement 2. 6) Do the authors find a correlation between the magnitude of neural responsivity and/or proportion of CS-responsive neurons with the CS-responses of the mice?
While there remained a significant sex difference in the amplitude of the currents after removing the non/minimal responders (males: 63±4 pA, n = 82; females: 41±5 pA, n = 54; unpaired t test, P<0.001), the lower proportion of cells responsive to acetylcholine suggested that male and female mice may have a different proportion of layer VI neurons containing nicotinic receptors.
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