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To jump the nuclear envelope barrier, we used a selective and reversible CDK1 inhibitor RO-3306 to control the G2/M transition of the cell cycle and increased the proportion of mitotic cells which have disappeared nuclear envelope during transfection.
Therefore, we examined for abnormalities in the proportion of mitotic phases upon Celastrol treatment.
Approximately 1000 cells from at least 3 different pups for both Lmna+/+and LmnaDhe/+ cultures were counted to determine the proportion of mitotic cells present.
These results indicate that upon inhibition of aPKCλ expression a higher proportion of mitotic spindles tend to align with the axis of polarity.
Similarly, in the O2 insensitive cell lines (HeyA8 and SKOV3) the proportion of mitotic cells remained unaltered regardless of O2 concentrations (Figure 3).
At 10°C, mutant strains exhibit a low proportion of mitotic cells with divided nuclei, indicating that anaphase does not readily occur.
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Contrary to the notion that high-grade cancers contain relatively large proportions of mitotic cells, we found that <2% of cells harbored mitotic spindles in the tumor samples examined (n=8 for each tissue).
Selective depletion of brat or prospero resulted in a significant increase in the proportion of pH3+ mitotic INPs in bantam mutant clones (Fig. 5C; representative images are in Fig. S8).
Interestingly, in A549EpoB40 cells ectopically overexpressing TUBB3 the proportion of multipolar mitotic spindles in response to drug treatments was significantly greater in comparison to the controls treated with any of the drugs.
It is a fact that malignant tumours can only arise in tissues with proliferating potential hence tissues with a large proportion of post mitotic cells such as the brain and the heart are rarely the seat of malignant tumours and the tumours derived from such tissues arise from cells with proliferating potential like the brain glia or the vascular endothelium [ 23, 24].
Following midbody staining with antibodies against Aurora B kinase, we observed a high proportion of post-mitotic cells with clearly condensed chromatin (Figure 4D).
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