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This study reveals that by using appropriate screening methods, a large proportion of memory B cells can be isolated that produce mAbs with HIV-1 neutralizing activity.
Among the cell donors, the proportion of memory CD4+CD25−CD45RO+ T cells expressing FOXP3 upon 96 hrs of pbCD3/sCD28 stimulation in presence of TGF-β was ∼20 40%.
Differences in the proportion of memory and naive B-cells in blood, LN and spleen between non-infected and SIV-infected animals were significant (p<0.05) at each time point (Figure 1C, D and E).
Studies in The Gambia [26] and Malawi [46] have shown that CMV infection is associated with a relatively high proportion of memory cells in both the CD4 and CD8 T-cell compartments, which accords with our findings.
In LN, the proportion of memory B-cells had decreased by factors of 1.3 by D14 p.i., 2.6 by D21p.i. and 2.2 by D28 p.i., respectively, whereas the percentage of naive B-cells increased by a factor of 2.7 from D14p.i.
Based on the previously established cut-off values (Figure 4), a significantly higher proportion of memory response was found in the subjects without active disease (5/11, 45.4%) compared with those with active TB (0/11, 0%) (p = 0.03) (Figure 5).
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Abdulahad et al reported that WG patients in remission exhibited an increase in the proportion of memory-like Treg (FOXP3+, CD25high, CD45RO+) among CD4+ T cells that are functionally defective [13].
The low CD4 T-cell numbers and higher proportion of memory-activated CD4 T cells may lead to restricted pathogen recognition in ICL.
The lack of memories from the first 3 or 4 years, due to childhood amnesia, affects the proportion of memories of the entire decade.
Only the average proportion of memories associated with places was significantly higher for weak (mean = 0.42) than strong (mean = 0.22) memories (t 9) = −3.56, P < 0.006).
The average proportion of memories associated with people did not differ significantly between weakly (mean = 0.40) and strongly (mean = 0.52) salient memories (t 9) = 0.906, P < 0.39).
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