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The proportion of invariant residues was determined empirically (0.119).
For ML analysis, the gamma shape parameter and the proportion of invariant sites were optimized.
The estimated gamma shape parameter (alpha) was 1.72 and the value of the proportion of invariant sites 0.04.
The nucleotide model used was a General Time Reversible model with 6 substitution rate parameters and gamma-distributed rate variation with a proportion of invariant sites.
The general time reversible model of nucleotide substitution was used, with gamma-distributed among-site rate variation, and a proportion of invariant sites.
Nucleotide trees were also created using RAxML according to the default parameters, again using the best tree and estimating the proportion of invariant sites.
The default substitution model was selected, assuming an estimated proportion of invariant sites and four gamma-distributed rate categories to account for rate heterogeneity across sites.
The Jones model for amino-acid substitution [34] + G (gamma distribution of rates with four rate categories) + I (proportion of invariant sites) was used.
In all cases the Jones-Taylor-Thornton (JTT) substitution matrix was used, the proportion of invariant sites estimated automatically and the best scoring tree used for visualization.
The general time reversible (GTR) model with a proportion of invariant sites of 0.2559 and a gamma shape parameter of 0.4721 was determined to be most appropriate.
I: proportion of invariant sites.
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