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Among these, the proportion of insertions that identified in one replicate but not the other (the replication failure rate) is less than 1%.
For each list, we then calculated enrichment for somatic L1 insertions versus what proportion of insertions were expected based on a random selection of 1x10 genomic points.
However, as expected, the gap percentage varied considerably with the relative proportion of insertions and deletions, with more gaps seen when the ratio of insertions to deletions was 1 1 and fewer when the ratio was 1 3, especially at low to medium substitution rates.
The proportion of insertions involved in clusters increased upon in vivo selection only in CD3+ cells differentiated from transduced HSC (44.8%, p < 0.05. Test on proportions with Holm Bonferroni correction) while it decreased in those derived from gene-corrected mature PBLs (34.0%, p < 0.001).
They explained the insertion bias as due to the influence of a single locus, O157-10, in a single PSPE, but in our study, on the contrary, when O157-10 was excluded from the analysis the proportion of insertions increased to 67%.
To some extent our data demonstrating significant overlap (about 70%) between late replicating loci in salivary gland polytenes and Kc cells, and a high proportion of insertions suppressed in eye cells in these regions, support this point of view on replication domain organization.
Similar(53)
Thus, the lower proportion of insertion mutants for the TE genes suggests that a significant portion of TEs might not be actively expressed.
For example, the proportion of insertion polymorphisms in CB4856 is 9942*10-8.
We were unable to identify any particular GO category responsible for the suppression of significant proportion of insertion.
By shotgun cloning the total mixture, it is possible to maintain the relative proportions of insertions from the starting material.
In summary, the Bayesian method is superior to the MP and ML ε methods under the gap coding approach, irrespective of the relative proportions of insertions and deletions in the alignment.
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