For each list, we then calculated enrichment for somatic L1 insertions versus what proportion of insertions were expected based on a random selection of 1x10 genomic points.
Among these, the proportion of insertions that identified in one replicate but not the other (the replication failure rate) is less than 1%.
They explained the insertion bias as due to the influence of a single locus, O157-10, in a single PSPE, but in our study, on the contrary, when O157-10 was excluded from the analysis the proportion of insertions increased to 67%.
However, as expected, the gap percentage varied considerably with the relative proportion of insertions and deletions, with more gaps seen when the ratio of insertions to deletions was 1 1 and fewer when the ratio was 1 3, especially at low to medium substitution rates.
The proportion of insertions involved in clusters increased upon in vivo selection only in CD3+ cells differentiated from transduced HSC (44.8%, p < 0.05. Test on proportions with Holm Bonferroni correction) while it decreased in those derived from gene-corrected mature PBLs (34.0%, p < 0.001).
To some extent our data demonstrating significant overlap (about 70%) between late replicating loci in salivary gland polytenes and Kc cells, and a high proportion of insertions suppressed in eye cells in these regions, support this point of view on replication domain organization.
The direct α for visitor-inserted pollinia (α dp ) is (2) α dp = α dT − 1 − r p α dc r p for α dT > α c 1 − r p, where α dT is the estimate of direct α for all insertions (both insertions through the bags and insertions into flowers of unbagged focal inflorescences), and r p is the proportion of insertions attributed to each visitor taxon.
considering that the position of entry in the insertion-sheet might influence the rate of mistakes (e.g., data entering errors from the last insertion field of a long insertion-sheet), we recorded the proportion of real insertion mistakes at the beginning and at the end of the insertion sheet.
