The most notable difference of the distributions was that the diploid F1-hybrid had a smaller proportion of expressed genes with a detectable expression bias.
silencing of the transgene occurred by a reduction of expression levels rather that a decrease in the proportion of expressing cells [39].
We next assessed the proportion of expressed genes carrying trimethylated H3K4 and/or H3K27 using an Agilent expression microarray platform by, first, defining present (expressed) and absent (not expressed) calls.
Analysis of Xist RNA by FISH revealed a high proportion of expressing cells in the GFP negative population (Figure 3B).
We found that 81% of H3K4me3 genes were expressed, representing an enrichment over the proportion of expressed Refseq genes identified in ZF4 cells regardless of the associated histone modification (57%; P<10−4 relative to the proportion of expressed RefSeq genes; Chi-square with Yates' correction; Figure 7A).
We found that, on average, only about 50% 60% of probe sets are called as present, which is similar to the overall proportion of expressed genes in most tissues [19].
A ratio was calculated from these areas to give the proportion of expressing cells.
G genes were generated, of them the proportion of expressed genes is k, and the proportion of differentially expressed genes is d of the G*k expressed genes.
The proportion of expressed env- and pol-derived sequences exceeded that of detected ERVs for each day, whereas the proportions of expressed pro-derived sequences were smaller than those found in the genome.
For each disease, we compared the proportion of expressed lncRNAs (>2 RPKM) in the whole genome (genome-wide, gray) with the proportion of expressed disease-specific lncRNAs (red), and tested for differences using two-tailed Fisher's exact test.
The relatively low proportion of expressed lizard OR genes may reflect the reduced role of olfaction in this species.
