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These tumours retain hallmarks of the normal epithelial terminal differentiation programme; however, proliferation is increased, the proportion of differentiated cells is decreased, and the spatial organisation of the cell layers is disrupted [1].
Expression of IGFBP-5.1 (Fig. 3C), IGFBP-5.2 (Fig. 3D) and IGFBP-6 (Fig. 3E) were high in mononuclear cells (2d) then declined as the culture matured and the proportion of differentiated myotubes increased (P<0.01).
The other two-locus combinations that exhibited a proportion of differentiated species pairs better than or equal to the best single locus were trnH-psbA+ITS1 (85.1%) and rbcL-a+matK (82.6%).
Like the full length mrTIMP-1 and its NT1 variant, 24 h of exposure to RXPO3R significantly reduced the length of neurites and increased the area of growth cones (Fig. 4B and C), without affecting cell viability or the proportion of differentiated neurons (not shown).
The trnH-psbA spacer when combined with either rbcL-a, rpoB2, or rpoC1 demonstrated the highest PCR primer success (100%, i.e., primers amplified for at least one if not both loci across all taxa) and the highest proportion of differentiated species pairs (87.5%; Table 4).
Total tau was expressed in a large proportion of differentiated WT RGs (Fig. 4E).
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Following injury, stem cells restore normal tissue architecture by producing the proper number and proportions of differentiated cells.
At 12 months of age, the vast majority of the CD8 T-cells of CMV-uninfected infants had been among the undifferentiated (CD27+, CD28+, CD62L+, perforin−, granzyme A−) subpopulations while those of CMV-infected subjects of equivalent age had shown substantial proportions of differentiated cells.
The intestine like other organs require stem cells in order to maintain adequate numbers and proportions of differentiated cells in the normal physiological state.
While this does not affect the analysis of the resulting differentiated (steady) states as evidenced by the tight match between the model and the measured proportions of differentiated cells [ 90] it could limit the model's ability to reproduce the transient dynamics of cellular differentiation.
However, a high proportion (∼80%) of differentiated ES cells targeted with the resulting SV40/Venus constructs showed YFP expression even without an enhancer (data not shown), indicating that the SV40 minimal promoter is leaky in this context and therefore inappropriate for our assay.
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