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Clearly the ability to detect DE is strongly associated with transcript length for RNA-seq regardless of the platform, statistical analysis procedure or overall proportion of differential expression.
In vitro experiments prove that variants in gene promoter regions effect rates of transcription and these variants may also lead to a significant proportion of differential allelic expression [ 46].
The relatively low proportion of differential SSH clones in the test set (3 out of 9) may reflect the choice of test primer pairs, which included only genes that were not previously annotated as pathogen-response dependent (Table 1).
B-statistic denotes the log-ratio of a locus to be differentially methylated over not being methylated, with B > 0 implying p > 0.5, dependent on the prior knowledge of the proportion of differential loci.
These two pathologies only share a small proportion of differential genes compared with normal muscle which indicates that the cell responds differently to various forms of mitochondrial dysfunction and that the changes that we have observed are specific to TK2 deficiency.
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It will be observed that still the former proportions of differential duties on East India and foreign coffee were retained during this period, and that, in consequence, the consumption of those kinds (especially of foreign, still subject to a very high duty) did not materially increase.
The proportions of differential biological processes in each module were computed.
A substantial proportion of the differential clones had no database matches either at the nucleotide or protein level.
In the Ctr vs. UG comparison, however, a smaller proportion of the differential regions were located within CGI shores (38%) and shelves (15%).
Within a given sample, we see that by sequentially examining more genomic dimensions at the DNA level, i.e. gene dosage, allelic status, and DNA methylation, we can explain a higher proportion of the differential gene expression changes observed.
We verified that the proportions of differentially methylated and differential expressed genes in each FEM were not strongly biased to one particular data type, thus confirming that FEM is effective in avoiding such bias (Additional file 1: Figure S2).
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