iPS cells expressed IKDR currents, and K+ channel block with tetraethylammonium (TEA) inhibited proliferation and viability, reduced the proportion of cells in the G0/G1 phase, and increased the proportion of cells in G2/M.
The consequence is that a considerable proportion of cells do not express human DHFR at levels that are sufficiently high to confer drug resistance.
With either approach, an 8 h induction of SCRaMbLE resulted in the highest proportion of cells with mean fluorescence above all un-SCRaMbLEd controls.
However, the shift in the proportion of cells in a mixed co-culture model for all experimental conditions was an effect of long-term culture.
However, the increased proportion of cells expressing GFAP has been observed in pure astrocyte cultures and not organotypic slice cultures46,52,53.
Control neurons showed a significant developmental shift to a larger proportion of cells expressing mature AP firing, reaching 90% of cells at 20 weeks in culture.
The proportion of cells transfected was monitored by fluorescence microscopy and the experiment was only continued for transfection rates greater than 50%.
For example, quantifying the proportion of cells in each phase of the cell cycle, including mitotic phases is very useful in the modern biological laboratory3.
(d) The proportion of cells in different cell cycle phases is displayed for WT and DKO cells based on single cell RNA-seq data (see Methods).
Moreover, the proportion of cells expressing CSC-associated markers was found enriched within residual tumors of colon cancer patients receiving chemoradiotherapy7.
This proportion of cells further increased to ∼53% (p < 0.05) and ∼58% after 48 and 96 h, respectively.
