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The sum-of-pairs score (SPS) is the proportion of aligned pairs of nucleotides/amino-acids from the true alignment that are also aligned together in the test alignment.
The alignment length coverage is calculated as the proportion of aligned length in the whole read.
The RFC score for each gene/non-coding region was calculated as the proportion of aligned bases that stay in frame.
The P. abelii species had an intermediate proportion of aligned ESTs (∼50% of the library and ∼30% of the distinct transcripts).
As expected, the proportion of reads aligned to the opossum genome is lower (since this species is evolutionarily more distant) with the proportion of aligned reads being 55% and 45% for PC and Bi, respectively.
The proportion of aligned segments varied across the six groups of quartets, from ~2,781 segments in the group containing BRASIL5, to ~3,672 in the group containing CIAT894.
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For such genes, the expression level from each homoeolocus was calculated by averaging the proportions of aligned reads with each homoeolocus-specific haplotype from all quantified regions, weighted by the total number of aligned reads in each region.
The C. jacchus, G. gorilla, M. mulatta, N. leucogeny, P. troglodytes species had relatively high-proportions of aligned ESTs (∼63% of the original ESTs and ∼41% of the distinct transcripts), and the M. murinus, O. garnettii, T. syrichta species had equally low-proportions of aligned ESTs (∼18% of the original library and ∼7% of the distinct transcripts).
We then performed Venn Diagrams between AGM and cDNA of the NHP species showing the highest proportions of aligned ESTs (M. mulatta, N. leucogeny, P. troglodytes, and H. sapiens), 31,005 of the 37,787 original ESTs and 8,909 of the 14,410 distinct transcrips could been aligned on at least one of the cDNA references.
Nearly all reads (98%) mapped against the Morex whole-genome shotgun sequence, although the number of reads mapped at several locations was two-fold higher than reads mapped only once.> -wrap-foot> Aligned reads : proportion of all aligned reads, reads aligned 1x and over 1x.
Ploidy estimation was carried out based on the proportion of all aligned reads aligning to each chromosome, calculated for each strain.
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