The number of cells in the VZ and SVZ was calculated and the proportion labeled with BrdU calculated.
In this technique, two different protein samples (control and diseased) and one internal control (mixture of control and diseased sample in equal proportion) are labelled with any of the three fluorophores: Cy2, Cy3, or Cy5.
Search data were normalized to a relative search volume (RSV) scale, where the highest search proportion was labeled 100 with lesser proportions scaled by how they relatively compared to the highest proportion.
Moreover, the proportion of β-galactosidase clusters at day 15 was identical to the proportion of labeled hepatocytes, strongly suggesting that the clusters originated from the labeled hepatocytes that were present in the liver before retrorsine administration.
Our analysis of labeling was qualitative because the proportion of labeled axons in each explant varied and fasciculation of small groups of axons, a varying proportion of which were MFas II-positive, precluded quantification.
Flow cytometry was used to measure the proportion of labeled MB49-GFP+ cells, while fluorescent microscopy was used to evaluate labeling on T24 cells.
Overall, our data clearly established that after retrorsine treatment the proportion of labeled hepatocytes was stable with the presence of numerous small clones of labeled hepatocytes.
In contrast, following 2-AAF treatment the proportion of labeled cells dropped and only rare large clusters of labeled hepatocytes were detected.
These models basically use two sets of data from the flow cytometry analyses: the proportion of labeled cells and the mean fluorescence intensities.
The proportion of labeled cells was highly variable between animals ranging from 0.2% to 8% when assessed at different time points.In these animals, we observed the presence of a small proportion of non-hepatocyte cells that were labeled.
