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† For each category (column), estimated non-redundant genes are calculated as proportion: assembled sequences/sequenced elements multiplied by total number significant.
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The combined treatment of CTGF and TGFβ-1 was found to increase the proportion of assembled primordial follicles and decrease the proportion of unassembled follicles (Figure 4B and 4C).
We estimated the size of the protein-coding portion of the nuclear genome in two ways: One based on the proportion of assembled contigs with protein hits, and the other based on the proportion of reads that contained BLASTX hits to a protein database.
As seen in previous studies [31], progesterone decreased the proportion of assembled follicles over 2 days.
The relative proportion of assembled follicles was compared across treatment groups (Figure 2A).
The ovaries treated with CTGF for two days were found to have an increased proportion of assembled follicles.
Over ten days of treatment only the combined treatment of CTGF and TGFβ-1 was found to cause an increase in the proportion of assembled follicles.
Interestingly, the combined treatment of both CTGF and TGFβ-1 was observed to increase the proportion of assembled follicles over ten days.
After 10 days of culture, CTGF was not found to increase the proportion of assembled follicles as was observed after two days of treatment.
Our results imply that when diversity is high, as in most natural viral communities [2], pre-fractionation of a community almost always improves the overall proportion of assembled sequences.
b Proportion of assembled transcripts matched by public ESTs.
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