Sentence examples for proper polymerase from inspiring English sources

After design and synthesis of a reversible terminator, first we need to find a proper polymerase to accept the nucleotide analog.

Posttranslational acetylation of these sites was recently shown to be required for proper polymerase pausing and regulation of two growth factor-regulated genes.

Due to the specificity of enzyme and the special structures of the reversible terminating nucleotides, it is difficult to get a proper polymerase to accept them with high efficiency and fidelity.

Taken together, we can postulate that the wild-type pol ι may require a low level of Mn2+ rather than Mg2+ as a metal for performing proper polymerase function for two reasons: (i) mediating efficient catalysis and (ii) facilitating tight DNA substrate binding, although the structural basis for such a scenario has not been revealed yet.

Proper polymerases were usually obtained after experimenting potential candidates through primer extension screenings [17,18].

Based on screening libraries, two approaches are applied to select the proper polymerases for the reversible terminators [17,18].

If no desired polymerase can be obtained in this screening, another approach can be taken to obtain the proper polymerases from mutation libraries constructed through rational design, "directed evolution" (random mutation), or semi-rational design (combination of rational design with directed evolution) [17].

We reasoned that, in Xenopus tadpoles, the human H1 promoter may not be recognized by the proper RNA polymerase (polymerase II rather than III), which would result in shRNAs with long single-stranded extensions that do not induce RNAi.

In spite of proper formation of polymerase complexes, the activity of RNPs including NLS mutations in PB2 was progressively reduced, in parallel to the decrease in their ability to interact with α-importin [10], as reflected in the activity of a CAT replicon in vivo (Fig. 2D).

A novel CTD modification of non-consensus repeats necessary for the proper regulation of polymerase pausing was recently discovered [ 20].

The polymerases of the B-family contain two enzymatic domain, namely, the N-terminal 3'-exonuclease domain that performs proofreading and the C-terminal polymerase proper domain [ 9, 10].