The phase field model can reproduce precise boundary kinetics and stress evolution provided that a thermodynamically consistent theory and proper expressions for model parameters in terms of physical material properties are employed.
DOI: http://dx.doi.org/10.7554/eLife.03573.016 To gain additional in vivo evidence that the DKC1 complex is required for the proper expression of genes critical for stem cell self-renewal, we performed loss-of-function studies using lentiviruses expressing two independent short hairpin RNAs (shRNAs) specifically targeting DKC1 in mouse D3 ES cells.
This could cause metabolic stress in the yeast cells, by limiting the availability of transcription factors required for proper expression of all the pathway proteins.
Although the mechanism of targeting of histone H3.3 deposition at specific loci in whole genome remains largely unclear, histone H3.3 has been considered as epigenetic regulator that is dispensable for proper expression of genes (Kato et al., 2015).
Third, we considered that sequence spacing within the ICR might be critical for proper expression of AK029869.
Additionally, we observed that Lsm12 is required for proper expression of Pbp4, as seen by microscopy and confirmed by western analysis (Figure 7A and B).
These data demonstrate that CaRF is required for proper expression of a least a subset of the gene products that neighbor the CaRF ChIP peaks.
This result is largely consistent with the promoter:GUS expression patterns (Figure 2, A F), indicating that the 1.6 kb promoter fragment likely contains all of necessary cis-elements for proper expression of OsPIE1.
As part of the TAC1 chromatin remodeling complex, Trithorax (Trx), the homolog of MLL1 in Drosophila, is required for proper expression of Hsp70 in response to heat shock [30].
Moreover, we show that Delg (CG6338), the Drosophila homologue to the alpha subunit of mammalian transcription factor NRF-2/GABP, is required for proper expression of most genes encoding mitochondrial proteins.
