Unfortunately, these systems offer low spatial resolution because of the high propensity for light scattering in tissue in the NIR.
Nine obps were down-regulated after light pulse stimulation in the female head; this pattern is likely to be related to the lower blood-feeding propensity of the mosquitoes after light pulse stimulation.
We then performed a series of assays to better understand the mechanism mediating the inhibition of blood feeding propensity by shorter light pulses.
To test whether A. gambiae blood feeding behavior is under circadian control we performed a time-course analysis of blood feeding propensity during a normal LD 12 12 light/dark cycle (12 h light, 12 h dark) and during a DD (dark-dark; continuous 48 hours) cycle to see whether the blood feeding propensity pattern would persist in absence of light.
Short light pulses (~2 5 min) in the dark phase can inhibit the blood-feeding propensity of mosquitoes momentarily in a clock independent manner, while longer durations of light stimulation (~1 2 h) can induce a phase advance in blood-feeding propensity in a clock dependent manner.
We further demonstrate that the propensity for cnidocyte discharge can be altered experimentally as a function of light intensity.
Collectively, our study sheds light on the propensity of BclXL to form amyloid-like fibrils with important consequences on its mechanism of action in gauging the apoptotic fate of cells in health and disease.
In contrast, gene silencing of light pulse regulated olfactory factors down-regulated the mosquito's propensity to feed on blood.
Thanks to the well-known propensity of positive muons to form Muonium, a light isotope of the hydrogen atom, the final stages of the absorption process can be probed.
The light:dark choice test is regarded as an unconditioned conflict paradigm, where the innate tendency for light avoidance conflicts with the propensity of mice to explore/escape novel places into which they are forced [6], [7].
Thus, in these calculations, it is critical to take into account the time resolution of the instrument and scattering propensity of the sample when calculating the fluorescence lifetimes and differentiating between light scattering and ultrafast quenching events.
