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The human ER+ MC cell lines, MCF7 and T47D, were obtained from the American Type Culture Collection ATCCC, Rockville, MD, USA) and were cultured as per ATCC propagation instructions.
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To construct the destination vector containing a toxic ccdB gene, ccdB survival™ competent cells (Invitrogen) were used for propagation according to the supplier's instruction.
Also, educating developers with energy efficient application development techniques including loop unrolling, branch optimization, dead store elimination, value numbering, code inlining, constant propagation, code motion, inter-procedural analysis, and instruction scheduling, greens smartphone operations [33].
In the 1659 instructions given by the Sacred Congregation for the Propagation of the Faith (known as the Propaganda Fide) to new missionaries to Asia, provisions were clearly made to the effect that adapting to local customs and respecting the habits of the countries to be evangelised was paramount: The Kangxi Emperor was at first friendly to the Jesuit Missionaries working in China.
When I am starting off seeds in pots, the instructions often say the surface must be covered to promote propagation.
Radio waves are propagated by a combination of three mechanisms: atmospheric wave propagation, surface wave propagation, and reflected wave propagation.
However, after instruction and discussion regarding features that are required to design a successful propagation plan (using our "How-To" Guide), agreement increased to over 80%% for subsequent use of the DSAAI.
Vegetative propagation has many advantages.
Or propagation of French culture?
"The propagation had gone well," she said.
This coconut was used for propagation purposes.
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