In contrast to aASCs, ASCs that were derived from subcutaneous tissue were able to propagate long term in culture because of their reduced ROS accumulation and reduced cell death.
However, primary human cancer cells are notoriously difficult to propagate long-term ex vivo, and primary tumor-derived fibroblasts appear to undergo phenotypic changes in short-term culture [6].
The reason for the dominance of CD4+ clones and the failure to propagate long-term CD8+ clones from these cultures is unclear.
This provided a considerable resource of human pluripotent stem cells (hPSCs) that could be propagated during long-term culture and yet be differentiated to a variety of lineages representative of the three embryonic germ layers [ 4].
You need large numbers to propagate them long-term.
It is through these neuroendocrine pathways that a self-perpetuating fear memory can propagate the long-term effects of early life trauma.
Yet another strategy used to locate renal stem cells has been to select them on the basis of their ability to propagate in long-term culture.
We aim to find major host- and microbe-related components that propagate or regulate these long term effects.
The non-mutagenized original clone was propagated to yield enough cells for RNA extraction and long term storage.
