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Additionally, we carried out a propagation experiment to investigate how far skylark songs are propagated in an open landscape corresponding to skylark habitat.
Although it is readily propagated by seed, experiments with in vitro propagation found Banksia menziesii to be more difficult than other species trialled.
Evolution experiments propagating bacterial populations in glucose- or phosphate-limited chemostats have been extensively developed to understand the dynamics of adaptive processes [ 19, 24- 26, 28].
E. coli XL1-Blue strain was used to propagate plasmids in cloning experiments.
This methodology has been successfully used to generate synthetic prion-like sequences that were able to form aggregates and propagate on in vivo experiments [ 39].
As sequencing yield and quality continues to increase and costs continue to decrease, the desire to conduct larger and more highly multiplexed experiments may propagate the problem of missing data further.
Escherichia coli strain DH5α was used for cloning experiments and propagated in LB medium.
These stroma cells were propagated and used for experiments between passages 8 and 24 as recently described.
Colonies were propagated from four transformation experiments (Table 1), and their purified DNA was short-read sequenced on an Illumina HiSeq to give nearly complete identification of every nucleotide in each genomic DNA sample (Table 2 and Figure S1).
The PK15 cells used in this study, for both viral stock production and virus-cell interaction experiments, were propagated in the H-MSM aproteic synthetic medium (R. L'Haridon, unpublished results) without serum.
Following the pioneering work Bateman (1959) and Mukai (1964), MA experiments involve propagating many replicate lines at very small effective population sizes so that the effect of natural selection is swamped out by that of genetic drift, allowing weakly selected mutations to accumulate randomly.
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