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Chromatograms were imported for proofreading with the vector NTI Advance 10 program (Invitrogen).
Chromatograms produced with the ABI Prism 3130XL DNA Sequencer (Applied Biosystems, PE Europe BV, Basel, Switzerland) were directly imported for proofreading with the vector NTI Advance 10 program (Invitrogen).
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The Ampli1™ WGA kit uses a polymerase with proofreading activity with a lower error rate (4.8 × 10−6) with respect to standard Taq DNA polymerases.
Complete applications well within deadlines to give yourself review time; it's astonishing how many people deliver material that has not been proofread, with an hour to go until the deadline.
Sequence reads were assembled with the CAP contig assembly program [ 33] and proofread with the BioEdit software [ 34].
Reaction products were fractionated on an ABI capillary sequencer (Applied Biosystems), and sequencing data were proofread with Chromas software (available from www.technylesium.com).
Intrinsic mechanism analysis revealed that during the continuous evolution process, the accumulation of genetically modified proofreading elements with mutator activities endowed the host cells with enhanced adaptation advantages.
But what if proofreading, along with copywriting and translation, could be offered akin to a Software-as-a-Service, API included?
DOI: http://dx.doi.org/10.7554/eLife.01519.004 10.7554/eLiFigure1 figureigure 1—figure supplement 2. The reported structures of proofreading domains with substrate-mimicking analogs.
This method, termed MDA (Multiple Displacement Amplification), takes advantage of the bacteriophage Φ29 DNA polymerase, a proofreading enzyme with high fidelity and potent strand displacement activities.
Comparison of these parameters from all known complex structures of proofreading domains with the structure presented in the current study places our structure in the same bracket as the other well-studied proofreading domains (Supplementary file 1C).
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