Sentence examples for proofreading function from inspiring English sources
Because reverse transcriptase lacks the "proofreading" function that most DNA-synthesizing enzymes have, many mutations arise as the virus replicates, further hindering the ability of the immune system to combat the virus.
Imbalanced dNTP pools usually lead to increased DNA mutations, DNA breaks and cell death by enhancing misincorporation and by inhibiting the proofreading function of DNA polymerases (Kumar et al., 2010; Zhang et al., 2014a, b).
Recently it has also been documented that an incorporation of a proofreading function to RT significantly increased the full-length content of cDNA up to 15-kb [42].
The high replicative activity of the virus, together with the lack of a proofreading function of the viral RNA polymerase, provides the basis for the significant genetic variability of HCV.
Our results show that the proofreading function of ε can be uncoupled from the SOS phenotypes.
Thus, the proofreading function of YbdB appears to be primarily directed at stalled aroyl- holoEntB adducts.
While polymerase and proofreading functions have been investigated extensively, the 5'-3' exonuclease activity has been subject to less scrutiny.
Pol α has limited processivity and lacks intrinsic 3′ exonuclease activity for proofreading functions (Kunkel et al. 1989).
Although we compared the results of using DNA polymerases with different proofreading functions (Q5 DNA polymerase from NEB and Platinum Taq DNA polymerase High Fidelity from Life Technologies) for the PCR amplification step of library construction, there was no significant difference when using barcode tags (Fig. 4).
Its mechanism of action (common to all aminoglycosides) results from binding to the 16S subunit of the bacteria's ribosomal RNA (rRNA) and disrupting the protein-proofreading function.
In addition, unlike the replicative polymerases, the lack of proofreading exonuclease function of the TLS polymerases permits them to continue DNA synthesis past many lesions, albeit with a lower fidelity.
