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Loss of proofreading by a replicative polymerase as Polδ increases spontaneous tumour development in mice [43].
A number of options exist for proofreading by polymerases.
Thus, despite generating a mutator effect itself, pol2-Y831A actually suppresses mutations resulting from loss of proofreading by Pol ε.
The interaction between the pol1-Y869A mutandon and the pol3-01 muthaton that inactivates proofreading by Pol δ is somewhat different.
(v ) The SM does not efficiently bind an improperly assembled, damaged, or non-cognate SNARE complex, exposing the complex to kinetic proofreading by Sec17 and Sec18.
To test if there is an effect of proofreading by Pol δ on the mutation rate in the pol2-Y831A mutant strain, we constructed strains with a combination of the pol2-Y831A mutandon and the pol3-01 muthaton that inactivates proofreading by Pol δ.
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