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We therefore conclude from the current study that HER2 trogocytosis is proof of target cancer cell elimination by ADCC.
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Improving survival rates of poor-prognosis childhood cancers via clinical introduction of new molecularly targeted anti-cancer therapeutics, through trials incorporating imaging for proof-of-target inhibition or predictive biomarkers, is a priority [ 1– 3], and will accelerate and improve the paediatric drug development process.
In the presence of carcinoembryonic antigen (CEA), a proof-of-concept target, target-analog is released to trigger the upstream CHA recycling circuit.
Nitrite was selected as a proof-of-concept target to trial their practical application.
Adenosine-5′-triphosphate (ATP), which is overexpressed on the extracellular surface of cancer cells besides the mitochondrial matrix, was used as a proof-of concept target molecule.
Escherichia coli O157 was used as a proof-of-concept target for quantitative analysis, and compared with the results for Staphylococcus aureus and Salmonella enterica to confirm the efficiency of the system.
Others include proof of the target: using donations to pay personal expenses.
This biomarker was chosen as a proof of principle target in this study.
Proof of tumour targeting in vivo in respective animal models is currently ongoing.
As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated.
These quantitative results were consistent with those qualitative results, giving a further proof of high targeting efficacy of the (MTX + PEG -CS-NPs to HeLa cells.
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