To validate the CBD as a tool for studying biofilm organization, three proof-in-principle experiments were carried out.
This software package was used to examine two additional CLSM data sets that were part of the proof-in-principle experiments described in this manuscript.
Collectively, these proof-in-principle experiments were designed to simultaneously illustrate important caveats in the interpretation of microscopy data but also to validate the CBD as a potential tool for the study of biofilm structure-function relationships.
These proof-in-principle experiments herald significant opportunities for utilizing 'omic' technologies in the rapid identification of traits with agronomic and nutritional importance that could serve to guide common bean breeding programs.
With regards to the underlying biology of microbial biofilms, these data illustrated two important points that were the focus of the proof-in-principle experiments carried out in this manuscript.
The utility of our assay is demonstrated in proof of principle experiments with ascorbic acid, where we find that vitamin C increases the fitness of esophageal squamous cells relative to Barrett's esophagus cells.
In proof of principle experiments we showed that the brain connectivity maps contain information on age and sex [ 59, 60]; we hypothesize that it can also be used for getting valuable insight into normal brain ageing and disease progression.
Since this baseline offset was not correctable using the normalization schema applied, these spectra were excluded from further analysis in the proof of principle experiments presented, yielding 100 patients available for analysis.
Following first proof of principle experiments, in the last couple of years EELS has been exhaustively used for the investigation of plasmon modes in single and coupled nanoparticles.
To assess the ability of anti-adhesive agents to modify drug penetration, we conducted proof of principle experiments in MCC, using the calcium chelating agent EGTA, which disrupts E-cadherin mediated cell adhesion.
This has been confirmed using proof of principle experiments in the mouse, where both copies of Apc have been removed from the kidney and only a small fraction of mice develop renal carcinoma.
