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In accordance with the previous reports, we identified pronounced induction of phospho-ERK 1/2 in doxycycline-treated MCF7-HER2 cells.
Consecutively addressing target cell-induced apoptosis, we found that K562 and ML2 cells provided pronounced induction of NK cell apoptosis, whereas, Jurkat cells had moderate effects.
Unlike FAD7/8-1, the FAD7/8-2 gene did not show pronounced induction by abiotic stress in response to either drought or cold temperature treatment.
After 2 h of exposure to 5 μM BPDE, a time point for which pronounced induction with B[a]P was observed, no significant upregulation of these genes by BPDE exposure was found (see Additional File 1, Figure S9).
Overexpression of VEGF-A, a prominent angiogenic and lymphangiogenic factor (Carmeliet et al., 1996; Nagy et al., 2002), caused pronounced induction of blood and lymph vessels at 4 days after injection.
We chose two clones (number 13 and 16, not shown) for further studies, as these clones provided pronounced induction of p16 expression with minimal 'leakage' of basal p16 expression in the absence of doxycycline.
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Pharmacological inhibition of primary ciliogenesis phenocopied the siRNA-mediated knockdown results and the inhibitory effect was more pronounced after induction of primary ciliogenesis through serum starvation.
The concentration profiles of reagent and products exhibited a pronounced initial induction period followed by a rapid increase of the reaction rate, suggesting an autocatalytic behaviour.
Gut inflammation and pronounced cytokine induction was observed in all animals infected with virulent S. Typhimurium strains.
The transcription of the principle sigma factor SigA, encoded by rpoD (sll0306, cluster 7), was significantly induced at 24-hr time-point, and the ECF-type (group 3) sigma-E factor, encoded by rpoE (sll0856, cluster 9), showed pronounced late induction.
Our data accords well with observations made in rat where peripherally active opioids are often ineffective if applied to healthy tissue but elicit pronounced antinociception upon induction of inflammation (Stein & Machelska, 2011; Stein et al, 1989).
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