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Apparently, the Trx fusion is ineffective in promoting solubility of r-Δgp160 antigen.
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This strategy promotes solubility enhancement of flavonoids and yields an improved active ingredient with innovative physical, physicochemical and functional characteristics.
A thioredoxin tag (TRX) was fused to mHDx-1 to promote solubility.
The extraction system used in our study promotes solubility of neutral and basic proteins as evident in the pIs of the identified proteins.
HDx-1 Q46 fused to a thioredoxin tag (TRX) to promote solubility (mHDx-1-TRX) was purified as reported previously [18] and exchanged into diluent (20 mM Tris-HCl, pH 7.4 containing 150 mM NaCl and 2 mM Ca2+) using a disposable PD10 desalting column (GE Healthcare).
The purpose of the Trx moiety was to promote solubility of the recombinant antigen and thus facilitate its in vivo biotinylation at the target site provided by BAP.
Commercially available methods for GAD65A and IA-2A are available as a radioimmunoassay with I-labeled GAD65 (truncated at the N-terminal end to promote solubility) and IA-2, respectively.
SRI practices may be promoting silicon solubility and uptake due to enhanced microbial activity, from greater root exudation and soil aeration, and from making more carbon available in the soil by providing compost.
It has been found that the presence of H2O in Bu4NClO4/PC plays a crucial role in improving the performance of Bu4NClO4/PC, such as increasing conductivity, decreasing viscosity, promoting CO2 solubility.
As the P3HT chains are intimately and permanently tethered on the DWCNT surface, a well-defined P3HT/DWCNT interface prevents the DWCNT from aggregating by promoting the solubility of P3HT-g-DWCNT nanocomposites in organic solvent.
For this reason, we sought a redox-insensitive element with a substantial solubility in forsterite, and a large enough ionic radius to substitute into its octahedral sites only unlike Al, which may also be important in promoting water solubility in olivine (Grant et al. 2007a).
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