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Such T-rich regions might generally function as cryptic bacterial promoters of variable efficiencies, depending on their sequence similarity to the wild type bacterial promoter.
Specifically, we placed all three reporters under the control of a medium strength RBS (BBa_B0032) and four different promoters of variable strength (in order of decreasing strength BBa_J23119, 101, 106, 115) on a low-copy vector (BBa_PSB4A5).
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Therefore, these Ugt1 and Ugt2 genes belong to a large class of genes whose expression patterns are determined by their respective promoters of multiple variable first exons [1].
The system variables include four types of rod promoters of varying blockage volume, bed particles of four sizes and four initial static bed heights.
The GA repeat regions, corresponding to the experimentally determined mouse promoter region, are of variable length across species [ 25].
However, while the activities of promoters were variable between species, both marsupial and human promoter regions show similar trends between comparable promoter regions.
The low CpG content promoters have a significantly higher fraction of variable CpG sites compared with the high CpG promoters (Fig. 4; P ≈ 0, chi-squared test).
The teams were of variable quality; while promoters sought the best cricketers, they still had to agree to terms.
In the breast cancer cell lines tested, each of the candidate promoters demonstrated variable relative luciferase activity between the different cell lines.
This observation corresponds to the previously reported phenomenon of variable expression of MMTV promoter-driven transgenes across mammary epithelium [ 19].
Additional file 5: Alignment of variable domains from SCAmpPs-3 and -4 promoters 130 bp 5′ to the transcription start.
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