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The results suggest that the strategy of combining the 2A peptide and double promoters efficiently mediates the co-expression of the four fluorescent proteins in pigs and is hence a promising methodology to generate multi-transgenic pigs by a single nuclear transfer.
While both promoters efficiently expressed p16AB and p16CD shRNA, there was a lower siRNA expression under hH1 promoter as compared to hU6 promoter (Additional File 2).
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In the same report, mutations of K99, 169, 181, 190 and 193 were demonstrated to induce IFN-β and NF-κB promoter efficiently.
In contrast, EF1α-eGFP+ cells showed similar levels of CD31 expression as EF1α -eGFP− cells, suggesting that the EF1α promoter efficiently drives eGFP expression in endothelial cells (Fig. 4F).
MLX was recruited normally to the wild type promoter, efficiently recruited to the ChoRE-b mutant promoter, and recruited to the ChoRE-a mutant promoter (albeit with a reduced efficacy); when both ChoREs were mutated, the MLX recruitment was completely impeded (Figure 8B).
For the GR, we have previously shown that complete methylation throughout each proximal GR promoter efficiently inactivates them [ 9].
Present results demonstrate that Ad5 viruses with the CMV promoter efficiently target postnatal SCs in explant cultures.
Up to now we were able to replace the viral E4 promoter efficiently with heterologous promoters of up to 1.5 kb in length.
We showed that insertion of an ICP4 expression cassette driven by the CMVIE promoter efficiently drove viral replication, whereas insertion of the ICP4 coding sequence (CDS) alone did not restore viral replication.
The goat uromodulin gene promoter efficiently targeted expression of GFP in the kidney of transgenic mice and more specifically in the epithelial cells of the TAL of Henle's loop.
Upon muscle injury, mice carrying the mutated C/EBP β promoter efficiently clear injured muscle from necrotic debris but display severe defects in muscle fiber regeneration, thus confirming that the persistence of inflammatory macrophages in damaged muscle of these mice is not sufficient for effective regeneration [ 64].
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