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This two-step transcriptional amplification (TSTA) method can boost promoters' activity, while maintaining cell specificity.
Overexpression of DR6-FL caused activation of IFN-α4 promoter in a dose-dependent manner, whereas there was little induction of IFN-α4 promoters activity when DR6-EX was overexpressed (Fig. 2F).
In this study, we demonstrated that spermine, a polyamine found in abundance in eukaryotic cells [76] alters L. interrogans promoters activity.
To assess the validity of the G. mellonella R-IVET screen, the rate of ermB gene excision by the ivi promoters activity was determined by extracting pCre2 derivatives from ivi clones and using them to electroporate strain Lox2 or Lox2ΔG.
To understand why the CDKN1A (promotermoter would be more sensitive to a small reductions in p53 than the HDM2 promoter in nutlin-3-treated cells, we propose a model based on the two following reports: First, it has been shown that CDKN1A and PIG3 promoters are bound by p53 and hdm2 and that the binding of hdm2 is associated with a reduction in the promoters' activity.
Similar(55)
In this assay, PGRE activated human COL1A2 promoter activity in a concentration-dependent manner.
The Tgfβ2 mRNA expression and promoter activity were examined.
Furthermore, SAL treatment increased the eap promoter activity.
This system allowed efficient systematic assays of promoter activity.
and its promoter activity was analyzed using particle bombardment.
Addition of RARβ/RXRβ further increased its promoter activity.
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