Sentence examples for promoter test from inspiring English sources

To evaluate the effect of sig gene overexpression on the activity of a particular promoter, the assay was performed as follows: The two-plasmid strain carrying the sig gene in the expression vector and a promoter in the promoter test vector pEPR1 was cultivated aerobically in 80 ml 2xYT medium at 30 °C.

The result suggests that, likely because of their length and count, cross-species conservation is required for de novo discovery on our promoter test sets.

These findings warrant further prospective clinical study to validate the additional benefit of developing an MS-MLPA based whole promoter test as an alternative or adjunct to the commonly used qMSP test.

"Promoter test region genes" and " Table S2.

As a promoter test vector we used the pILA plasmid [ 43] into which ~300 bp long promoter fragments were cloned as transcriptional fusions with the luxAB genes.

To verify these inverted repeats as binding sites for CysR, several promoter test plasmids based on the promoter-probe vector pRIM2 [ 31] were constructed.

Here, we report new features added to the Gateway-compatible vector pGWB533 for promoter testing with the reporter gene encoding β-glucuronidase (GUS).

In no case did we observe binding at any promoter tested under repressed conditions, and we present here data after four hours of derepression.

Thus, attention has been given toward promoter testing and discovery for switchgrass genetic engineering [ 60, 61].

Therefore the fluorescence of the TEF1 promoter fusions were used to normalize exposure times for GFP fluorescence between backgrounds for the other promoter tests.

Thus, the Sp-1 site between -79 and -70 bp needs to be mutated and the activity of the monkey 20α-HSD mutant promoter tested.