However, using multiple promoters to increase the level of ferritin expression in rice seeds did not significantly increase the Fe concentration compared to expression driven by a single endosperm-specific promoter (Table 1; Qu et al. 2005).
PCR amplifications were performed on immunoprecipitated chromatin using primers to amplify specific regions in the FLT1 promoter (Table S2).
The plasmid pIO5 carries the genes for a mutant RNR protein under control of an IPTG-inducible promoter (Table 1).
Seven of the 8 mutations statistically significant in both case-breed analyses were in the equine OAS1 promoter (Table 3).
Adcy7 expression is abundant in macrophages, stimulated by IFNγ, and associated with STAT-1 binding to the Adcy7 gene promoter (Table S2).
VEGF and CXCR4 promoter deletion mutants were generated using forward primers containing a 5' KpnI restriction site and 3' end complementary to the promoter (Table S2).
The absence of genomic DNA contamination was verified by quantitative PCR with primers for the MtMADS1 gene intron and the EFD promoter (Table S8).
qPCR assays were designed, using the Roche Universal ProbeLibrary Assay Design Center, for the mitochondrial genome overlapping two neighbouring genes (tRNA leucine 1 and NADH dehydrogenase 1) and the nuclear genome using the β2-adrenergic receptor gene promoter (Table S1).
The xrep-1a or xrep-1b promoter was amplified with the primers XREP-1abp_HindIII_For and XREP-1ap_cDNA_Rev (xrep-1a promoter) or XREP-1abp_HindIII_For and XREP-1bp_cDNA_Rev (xrep-1b promoter) (Table S2) by PCR from the N2 genomic DNA.
Interestingly, the analysis found that some of the intragenic miRNA genes may be transcribed by their own promoter (Table 3), which was also observed in the recent analysis by Ozsolak et al. [15].
The plasmid pML005, derived from PUC18 with Cm cassette in exchange of bla gene and a mutation in the ColE1-type replication promoter (Table S1) which was previously shown to confer a stability to the plasmid in L. pneumophila [18], was used to clone tolC gene under the control of a constitutive promoter (Pkan, constitutive promoter of kan gene from plasmid pCDP05).
