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For construction of a lentiviral vector expressing miR-122, the human H1 promoter (position -220 to +1) was amplified by PCR and subcloned into pBS as described [35].
Given the tightly constrained promoter position relative to the TSS (usually between 7 to 10 nucleotides) promoter identification is straightforward once the TSS has been experimentally determined.
For modification with KMnO4 a DNA-fragment containing only the bolA P1 promoter (position -58 to 49 relative to the start site) was prepared by cleavage of pUC18-bolA1 with EcoRI and BsaAI.
The same is also true for mutations occurring at inhA promoter position -34.
Individual acetylation sites are differentially regulated dependent on the stimulus and the promoter position.
Highly individual patterns, depending on the promoter position, were observed for the tested acetylation sites.
Similar(35)
The insertion of different numbers of EREs in three alternative promoter positions and its effect on reporter gene expression were assessed.
To evaluate whether genotypes of interleukin (IL -6 gene promoter posIL -6s -174 and -572 are associated with histologenechorioamnionitis and neonatal inflammatory disease in promoterinfants.
These spatial constraints on promoter positioning may reflect an RNA polymerase-independent mechanism to target adjacent gene segments for chromatin remodeling events that facilitate rearrangement.
These vectors also harbor a bacteriophage T7 promoter positioned upstream of the ZFN coding sequence.
Convincingly, the hTERT promoter region is embedded in a CpG island (positions −1100 to +1500), and this region is mostly hypermethylated by specific DNA methyltransferases (DNMTs) in cancer cells except a short region in the hTERT core promoter (positions −279 to +5) [21].
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