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This may be important for the expression of a photochemically internalised transgene under a specific promoter control.
RNA polymerase II transcription complexes reveal the basis of abortive initiation and its role in promoter control.
The LIP2 isoenzyme gene from Candida rugosa has been completely synthesised and functionally expressed under the AOX1 promoter control in Pichia pastoris.
This work explores some of the possibilities to replace human cytomegalovirus (CMV) core and/or enhancer promoter control elements to create new expression vectors for use with fish.
When its recognition site of seven residues is engineered into target proteins and NIa protease is expressed under tight promoter control, substrates can be conditionally processed while other cellular proteins remain unaffected.
We describe methodology for using a red light and phytochrome dependent system to induce transcription of genes under GAL1 promoter control, as well as blue light/cryptochrome dependent systems to control transcription of genes under GAL1 promoter or LexA operator control.
Additionally, the original pGL3 was used as SV40 promoter control.
GAPDH promoter control primers were (forward) 5'- GGCTGCGGandAGTTGTTGAGGA-3' and (reverse) 5'- CTGAGTCCTATCCTGGGAACCAT-3'.
We are currently testing these two models using the GFP gene cloned under the gal promoter control.
Significant positive signals were not detected for the other Dnmt1 promoter regions under consideration or for the β-actin promoter control.
Expression of all three genes, under tuber-specific promoter control, results in tubers with a deep yellow ("golden") phenotype without any adverse leaf phenotypes.
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