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These data show that a large proportion of unfused GrB was retained in an intracellular compartment likely representing the ER, while GST and MBP domains both promoted secretion of GrB.
For instance, PKD1 has been involved in secretion of matrix metalloproteinase-2 and -9 from prostate cancer cells [61] or of insulin [62]; PKD2 was shown to regulate hypoxia-induced VEGF-A secretion from pancreatic tumor cells [63] or secretion of chromogranin A from neuroendocrine tumor cells [64]; and PKD3 promoted secretion of cholecystokinin-mediated pancreatic amylase [65].
DPN promoted secretion of several critical growth factors (P < 0.001).
15 showed that 3-D culture using collagen scaffolds promoted secretion of vascular endothelial growth factor (VEGF) in MCF-7 cells.
Initial studies suggested that extracellular recombinant HMGB1 promoted secretion of inflammatory cytokines (TNFα, IL-1β, and IL-6) by macrophages [ 91].
In contrast, TNF promoted secretion of MMPs but IL-17 did not augment TNF, indicating that IL-17 acts via an independent mechanism.
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Senescent cells secrete HMGB1 promoting secretion of inflammatory cytokines (TNFα, IL-1β and IL-6) by macrophages [ 55].
These studies indicate that while the SP is very important in promoting secretion of the scFv, it had little influence on increasing scFv secretion levels even when both the TEV and the KDEL sequences significantly increased overall protein levels.
More importantly, AMF inoculation ameliorated inhibitory effects of ZnONPs by promoting secretion of glycoprotein called glomalin a potent metal chelator within the rhizosphere, which significantly reduced (by almost half) Zn uptake by root and subsequent translocation to the shoot.
Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin.
Moreover, using cocultured cells (DPSCs/CCN3 and DPSCs) in vitro and the cocultured cells-poly (lactic-co-glycolic acid) (PLGA) scaffold complex in vivo, we demonstrated that CCN3 was capable of promoting mineralization in a non-cell autonomous manner through promoting secretion of BMP2.
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